Journal: Molecular carcinogenesis

Article Title: IL-33 Promotes Growth and Liver Metastasis of Colorectal Cancer in Mice by Remodeling the Tumor Microenvironment and Inducing Angiogenesis

doi: 10.1002/mc.22491

Figure Lengend Snippet: IL-33 enhanced tumor growth. (A) Proliferation of MC38-Vector and MC38-IL33 cells in tissue culture. (B and C) Weights (grams) of primary tumors derived from MC38-Vector or MC38-IL33 cells 6 weeks after cecal implantation (B) or subcutaneous injection (C). ***P <0.001. (D) Incidence of primary tumor growth in cecum of C57Bl/6 mice implanted with MC38-Vector or MC38-IL33 cells (n =20). (E) H&E images (400×) and primary cecal tumor sections stained with anti Ki67 and cleaved caspase-3 antibodies. (F) Immunoblot analysis of total protein extracts from MC38-Vector and MC38-IL33 clones with antibodies against PCNA and cleaved caspase-3. (G) Serum levels of IL-33 in mice bearing MC38-Vector or MC38-IL33 tumors analyzed by ELISA.

Article Snippet: Endogenous peroxidases were blocked with 3% H 2 O 2 for 15 min. Nonspecific epitopes were blocked with normal horse serum (Jackson ImmunoResearch, West Grove, PA) for 1 h. The sections were incubated overnight at 4°C with antibodies against one of the following proteins: LOX, MMP9, MMP2, LYVE1, VEGF, and S100A8 (all from Abcam, Cambridge, MA), S100A9, IL-33 (R&D Systems, Minneapolis, MN), cleaved Caspase-3 (Cell Signaling Technology, Danvers, MA), Ki67 (OriGene Technologies, Rockville, MD).

Techniques: Plasmid Preparation, Derivative Assay, Injection, Staining, Western Blot, Clone Assay, Enzyme-linked Immunosorbent Assay

Journal: Archives of Gynecology and Obstetrics

Article Title: rHGF interacts with rIGF-1 to activate the satellite cells in the striated urethral sphincter in rats: a promising treatment for urinary incontinence?

doi: 10.1007/s00404-018-4930-2

Figure Lengend Snippet: In Ki-67 staining, all nuclei were stained red with PI. The satellite cells expressing Ki67 were stained blue with Ki67 antibody. In MYH3 and NCAM staining, all nuclei were stained blue with DAPI. The satellite cells were stained red with MYH3 and NCAM antibody. All of the images are merged and show co-localization

Article Snippet: To detect Ki67, after cultivated with BSA for 45 min, the sections were incubated overnight at 4 °C with the Ki67 antibody (CST; 12075S), Propidium bromide (PI) was applied to the sections for 30 min to identify nuclei.

Techniques: Staining, Expressing

Journal: Cancer Research Communications

Article Title: Novel Syngeneic Cell Lines for Studying High-Risk BRAF V600E -Driven Colorectal Cancer In Vivo

doi: 10.1158/2767-9764.CRC-25-0599

Figure Lengend Snippet: All NaJa cell lines successfully engraft into syngeneic immunocompetent C57BL/6N mice and recapitulate their individual 2D morphologies in vivo . A, Schematic overview of the PVI of NaJa cells ( n = 2 NaJa-D, n = 10 NaJa-F, and n = 3 NaJa-G). B, Left, livers showing metastatic nodules 4 weeks after injection (black arrows) and invasive growth in the pancreatic tissue (blue circles). Scale bar, 1 cm. Hematoxylin and eosin: Histology showing that all NaJa cell lines establish tumors (pale blue) in the liver parenchyma (pink) with a differentiation state matching their epithelial or mesenchymal in vitro morphology. Right, IHC and immunofluorescence (IF) staining of proliferation marker Ki-67 (left) and intestinal differentiation markers CDX2 (middle) and E-cadherin (right). L, liver; T, tumor. Scale bar (overview), 500 μm, scale bar (zoom, IHC, and IF), 50 μm. C, Quantification of Ki-67 and CDX2 IHC staining in NaJa cell–induced liver metastases. Each dot represents an individual tumor; different shapes indicate individual tissue sections, and colors denote individual mice. Data are shown as the median ±95% confidence interval (CI).Statistical significance was assessed using the Kruskal–Wallis test followed by Dunn multiple comparison test. **, P < 0.01; ****, P < 0.0001. D, IHC staining of ERK and its downstream target DUSP6. Red arrows indicate examples of nuclear ERK staining. L, liver; T, tumor. Scale bar, 50 μm. [ A, Created by J. Traichel in BioRender. Brummer, T. (2025) https://BioRender.com/8ogg64c .]

Article Snippet: The formalin-fixed, paraffin-embedded (FFPE) sections underwent standard processing and staining procedures using the following primary antibodies directed against E-cadherin (1:50, #610181, BD Laboratories; RRID: AB_397580) and Ki-67 (1:400, #9129, Cell Signaling Technology; RRID: AB_2687446).

Techniques: In Vivo, Injection, In Vitro, Immunofluorescence, Staining, Marker, Immunohistochemistry, Comparison

Journal: Heliyon

Article Title: Genetically engineered mouse model of HPV16 E6-E7 with vaginal-cervical intraepithelial neoplasia and decreased immunity

doi: 10.1016/j.heliyon.2024.e29881

Figure Lengend Snippet: Intraepithelial neoplasia was promoted in the engineered mice treated with estrogen A, Image of cervical-vaginal tissue of the experimental group ( HPV16-E6-E7 -engineered mice treated with estrogen) and control group (wild-type C57BL/6 N mice). B, Pathological section exhibiting hyperplasia in the experimental group. C and D, Immunohistochemistry showed positive expression of Ki67 and p16 in the experimental group. Scale bar: 100 μm or 200 μm.

Article Snippet: Other materials used included as follows: DNA extraction kit (Cwbio, Technology Co., Jiangsu, China.), PE anti-mouse CD3 antibody (100308, Armenian Hamster lgG), CD8a (100722; BioLegend), red cell lysis solution (C3702; Beyotime), type I collagenase (Sigma, USA), DNase I (Sigma, USA), Ki67 rabbit polyclonal antibody (TA336650; OriGene), P16 rabbit monoclonal antibody (TA592012; OriGene).

Techniques: Control, Immunohistochemistry, Expressing

Journal: Oncology reports

Article Title: Elevated expression of coactivator-associated arginine methyltransferase 1 is associated with early hepatocarcinogenesis.

doi: 10.3892/or.2013.2651

Figure Lengend Snippet: Figure 2. Immunohistochemistry of CARM1 and GST-P. (A) Sections derived from rats treated with DEN for 12 weeks were stained with anti‑CARM1 antibody (right) and anti-GST-P antibody (middle). H&E staining (left) is shown (x40). (B) Higher magnification of the rectangle in A (x400). (C) Percentage of CARM1-positive cells, (D) expression level of CARM1 in the nucleus, and (E) percentage of Ki67-positive cells. Values are presented as the means ± SE (n=3). Significant differences were evaluated with Tukey's multiple comparison test. **p<0.01 and ***p<0.001 compared to normal liver. #p<0.05, ##p<0.01 and ###p<0.001 compared to GST-P-negative cells.

Article Snippet: Paraffin-embedded specimens were sectioned (3 μm) and stained with rabbit anti-rat GST-P antibody (MBL, Nagoya, Japan), CARM1 antibody (Millipore, Billerica, MA, USA) or Ki67 antibody (SP6; Acris Antibodies GmbH, Herford, Germany), and then with anti-mouse or anti-rabbit secondary antibody and avidin-biotin complex (Vectastatin Elite ABC kit; Vector Laboratories, Burlingame, CA, USA), and binding sites were visualized with diaminobenzidine.

Techniques: Immunohistochemistry, Derivative Assay, Staining, Expressing, Comparison